Leukocyte-Mimetic Liposomes Penetrate Into Tumor Spheroids and Suppress Spheroid Growth by Encapsulated Doxorubicin

As leukocytes can penetrate into deep regions of a tumor mass, leukocyte-mimetic liposomes (LM-Lipo) containing leukocyte membrane proteins are also expected to tumors by exerting properties those proteins. The aim the present study was examine whether LM-Lipo, which were recently demonstrated actively pass through inflamed endothelial layers, spheroids, and investigate potential LM-Lipo for use as an anticancer drug carrier. We prepared via intermembrane protein transfer from human leukemia cells; onto determined Western blotting. significantly high association with lung cancer A549 cells compared plain liposomes, contributed effective anti-proliferative action encapsulated doxorubicin hydrochloride (DOX). Confocal microscopic images showed that but not could efficiently spheroids. Moreover, DOX-encapsulated suppressed spheroid growth. Thus, transferred retained their unique function, allowed efficient penetration similar leukocytes. In conclusion, these results suggest be useful tumor-penetrating delivery system treatment.